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SENSISpec ELISA Lupin 96 Wells
The SENSISpec Lupin ELISA represents a highly sensitive detection system for lupin and is particularly capable of the quantification of residues in sausage, bakery products, potato products, ketchup and juices. Due to high cross-reactivity the test is suitable for the detection of all commonly used lupin species.
|
SKU |
HU0030011 |
|---|---|
|
Test principle |
Sandwich enzyme immunoassay |
|
Format |
Microtiter plate |
|
Incubation Time |
60 min (20/20/20 min) |
|
Standards |
0; 2, 5; 15; 30 ppm |
|
Detection |
450 nm (HRP + TMB) |
|
Detection limit (LOD) |
Sausage 0.2 ppm Bread 0.3 ppm Orange juice 0.7 ppm Ketchup 0.1 ppm Croquette 0.2 ppm |
|
Quantitation Range |
2 - 30 ppm |
|
Cross-reactivities |
Chick pea 0.0003% Lentil 0.0004% Soy flour, unroasted 0.07% Soy flour, roasted 0.0009% |
|
Shelf Life (months) |
13 |
|
Storage |
2–8°C |
Lupin belongs to the legume family. With about 35-45% the fraction of proteins in lupin seed is very high. Some of these proteins, predominantly those in the α-, β- and γ-Conglutin fractions are known for being allergenic, due to cross-reaction to allergens of soy and peanut. Because of its convenient nutritional properties the use of lupin flour as a food ingredient is highly increasing. Lupin is often used in dietary products, especially for milk allergic people, vegetarians and patients with celiac disease. In food production three (sweet) lupin species are commonly used: Lupinus albus, Lupinus angustifolius and Lupinus luteus.
For lupin allergic persons hidden lupin allergens in food are a critical problem. Already very low amounts of lupin can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Cross-contamination, mostly in consequence of the production process, is often noticed. This explains why in many cases the existence of lupin residues in food cannot be excluded. For this reason sensitive detection systems for lupin residues in foodstuffs are required.
The SENSISpec Lupin ELISA represents a highly sensitive detection system for lupin and is particularly capable of the quantification of residues in sausage, bakery products, potato products, ketchup and juices. Due to high cross-reactivity the test is suitable for the detection of all commonly used lupin species.
- SENSISpec ELISA Lupin 96 Wells
- SENSISpec ELISA Lupin 48 Wells
The SENSISpec Lupin ELISA represents a highly sensitive detection system for lupin and is particularly capable of the quantification of residues in sausage, bakery products, potato products, ketchup and juices. Due to high cross-reactivity the test is suitable for the detection of all commonly used lupin species.
