IAC
IAC for Fumonisins B1,B2,B3
1. Purpose:
The immunoaffinity column can selectively adsorb fumonisin (FB1, FB2, FB3) from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the column can be directly used for LC- MS/MS analysis after concentrating with nitrogen gas and resuspended or for HPLC analysis after derivatization.
Affinity columns can be used in combination with HPLC or LC- MS/MS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.
2. Overview:
Fumonisin is a metabolite produced by Fusarium fungi. It has acute and chronic toxicities and is species-specific and organ specific. Studies have found that high concentrations of fumonisin can cause acute species- specific toxicity symptoms in many domestic and laboratory animals, such as equine leukoencephalomalacia, porcine pulmonary edema, and liver and kidney diseases in sheep. The toxin has also been found to be related to esophageal and liver cancer in humans.
3. Principle:
The basis of the measurement is the antigen-antibody reaction. Antibodies are connected to the column and the fumonisin in the sample is extracted, filtered, and diluted, and then passed slowly through the Ochratoxin A immunoaffinity column. The toxins bind to the antibodies in the column and the immunoaffinity column is then washed to remove other unrelated substances that have not been bound. Fumonisin is then eluted with methanol and injected into an analytical instrument for detection.
4. Components of the kit:
Each kit contains fumonisin immunoaffinity columns of various specifications and 1 instruction manual.
The immunoaffinity column can selectively adsorb fumonisin (FB1, FB2, FB3) from the sample solution, thereby having a highly targeted purification effect on the sample. The sample solution that has been purified by passing through the column can be directly used for LC- MS/MS analysis after concentrating with nitrogen gas and resuspended or for HPLC analysis after derivatization.
Affinity columns can be used in combination with HPLC or LC- MS/MS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.
Order No.: HCM0825B
Catalog No. Product Name Column Capacity (ng) Specification
HCM0125B/50B IAC for Aflatoxins Total B1,B2,G1,G2 300 3mL, 25T/50T
HCM0225B/50B IAC for Aflatoxins Total B1,B2,G1,G2,M1,M2 AFT B1,B2,G1,G2: 300 AFT M1: 100 AFT M2: 100 3mL, 25T/50T
HCM0325B/50B IAC for Aflatoxin B1 300 3mL, 25T/50T
HCM0525B/50B IAC for Zearalenone 2000 3mL, 25T/50T
HCM0625B/50B IAC for Deoxynivalenol 2000 3mL, 25T/50T
HCM3025B/50B IAC for Deoxynivalenols (DON/3-ADON/15-ADON) DON: 2000 3-ADON: 1000 15-ADON: 2000 3mL, 25T/50T
HCM0725B/50B IAC for Ochratoxin A 100 3mL, 25T/50T
HCM0825B/50B IAC for Fumonisins B1,B2,B3 5000 3mL, 25T/50T
HCM0925B/50B IAC for T-2 Toxin 2000 3mL, 25T/50T
HCM2025B/50B IAC for T-2,HT-2 Toxin 2000 3mL, 25T/50T
HCM2125B/50B IAC for Citrinin 500 3mL, 25T/50T
HCM2225B/50B IAC for Sterigmatocystin 600 3mL, 25T/50T
HCM3825/50 IAC 2-in-1 Aflatoxin B1/Deoxynivalenol IFU 3mL, 25T/50T
HCM3325B/50B IAC 2-in-1 Zearalenone/Fumonisins IFU 3mL, 25T/50T
HCM3525B/50B IAC 2-in-1 Zearalenone/Deoxynivalenol IFU 3mL, 25T/50T
HCM3725B/50B IAC 2-in-1 Aflatoxins (B1,B2,G1,G2)/Ochratoxin A IFU 3mL, 25T/50T
HCM4825B/50B IAC 2-in-1 Aflatoxin B1/Ochratoxin A IFU 3mL, 25T/50T
HCM5925B/50B IAC 2-in-1 Aflatoxin (B1,B2,G1,G2,M1,M2)/Ochratoxin A IFU 3mL, 25T/50T
HCM6025B/50B IAC 2-in-1 Zearalenone/Deoxynivalenols (DON,3-ADON, 15-ADON) IFU 3mL, 25T/50T
HCM6125B/50B IAC 2-in-1 Aflatoxins (B1,B2,G1,G2)/Zearalenone IFU 3mL, 25T/50T
HCM3125B/50B IAC 3-in-1 Aflatoxin B1/Zearalenone/Deoxynivalenol IFU 3mL, 25T/50T
HCM4125B/50B IAC 3-in-1 Aflatoxins (B1,B2,G1,G2)/Zearalenone/Deoxynivalenol IFU 3mL, 25T/50T
HCM6825B/50B IAC 3-in-1 Aflatoxins (B1,B2,G1,G2)/Zearalenone/Ochratoxin A IFU 3mL, 25T/50T
HCM4725B/50B IAC 3-in-1 Aflatoxin B1/Zearalenone/Ochratoxin A IFU 3mL, 25T/50T
HCM4925B/50B IAC 3-in-1 Zearalenone/Deoxynivalenol/Fumonisins IFU 3mL, 25T/50T
HCM6225B/50B IAC 3-in-1 Zearalenone/Deoxynivalenol/Ochratoxin A IFU 3mL, 25T/50T
HCM6525B/50B IAC 3-in-1 Aflatoxin B1/Zearalenone /T-2 Toxin IFU 3mL, 25T/50T
HCM3225B/50B IAC 4-in-1 Aflatoxin B1/Zearalenone/Deoxynivalenol/Ochratoxin A IFU 3mL, 25T/50T
HCM3625B/50B IAC 4-in-1 Aflatoxin B1/Zearalenone/Deoxynivalenols (DON,3- ADON,15-ADON)/Fumonisins IFU 3mL, 25T/50T
HCM6625B/50B IAC 4-in-1 Aflatoxins (B1,B2,G1,G2)/Ochratoxin A/Fumonisins/ Sterigmatocystin IFU 3mL, 25T/50T
HCM3110 IAC Mycotoxins 6-in-1 Combo IFU 6ml, 10T HCM4210 IAC Mycotoxins 7-in-1 Combo IFU 6mL,10T
Sample type: Grain,oil,feed,etc